Western Blot of Rabbit Anti-RFX5 antibody (GTX27542). Raji B cell nuclear extract lysates. Load: 35 microg per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4oC. Secondary antibody: HRP Goat-a-Rabbit IgG at 1:5,000 for 45 min at RT.
Western Blot of Rabbit Anti-RFX5 antibody (GTX27542). Raji B cell nuclear extract lysates. Load: 35 microg per lane. Primary antibody: RFX5 antibody at 1:2,500 for overnight at 4oC. Secondary antibody: HRP Goat-a-Rabbit IgG at 1:5,000 for 45 min at RT.
RFX5 antibody
GTX27542
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, Other Application
Product group Antibodies
ReactivityHuman
TargetRFX5
Overview
- SupplierGeneTex
- Product NameRFX5 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ELISA: 1:5000-1:25000. EMSA: 0.5-1 microL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5993
- Target nameRFX5
- Target descriptionregulatory factor X5
- Target synonymsDNA-binding protein RFX5; regulatory factor X 5; regulatory factor X, 5 (influences HLA class II expression)
- HostRabbit
- IsotypeIgG
- Protein IDP48382
- Protein NameDNA-binding protein RFX5
- Scientific DescriptionRFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex, which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203