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Western Blot Positive WB detected in: Rat liver tissue, Mouse liver tissue All lanes: RGN antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 25 kDa Observed band size: 34 kDa
Western Blot Positive WB detected in: Rat liver tissue, Mouse liver tissue All lanes: RGN antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 25 kDa Observed band size: 34 kDa
Western Blot Positive WB detected in: Rat liver tissue, Mouse liver tissue All lanes: RGN antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34, 25 kDa Observed band size: 34 kDa

RGN Antibody

CSB-PA623001LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRGN
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Overview

  • Supplier
    Cusabio
  • Product Name
    RGN Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9104
  • Target name
    RGN
  • Target description
    regucalcin
  • Target synonyms
    GNL, HEL-S-41, RC, SMP30, regucalcin, epididymis secretory protein Li 41, gluconolactonase, senescence marker protein 30
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15493
  • Protein Name
    Regucalcin
  • Scientific Description
    Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities (By similarity).
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Regucalcin using anti-Regucalcin antibody (A05900-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: human SMMC-7721 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Regucalcin antigen affinity purified polyclonal antibody (Catalog # A05900-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Regucalcin at approximately 33, 43KD. The expected band size for Regucalcin is at 33KD.
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