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Western Blot analysis of 3T3 cells using RGS12 Polyclonal Antibody
Western Blot analysis of 3T3 cells using RGS12 Polyclonal Antibody
Western Blot analysis of 3T3 cells using RGS12 Polyclonal Antibody

RGS12 Antibody

CSB-PA040214
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRGS12
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Overview

  • Supplier
    Cusabio
  • Product Name
    RGS12 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6002
  • Target name
    RGS12
  • Target description
    regulator of G protein signaling 12
  • Target synonyms
    regulator of G-protein signaling 12, regulator of G-protein signalling 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14924
  • Protein Name
    Regulator of G-protein signaling 12
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RGS12 using anti-RGS12 antibody (A07317-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS12 antigen affinity purified polyclonal antibody (Catalog # A07317-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGS12 at approximately 156 kDa. The expected band size for RGS12 is at 156 kDa.
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