Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![RGS4 antibody [HL1522] detects RGS4 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX636996/GTX636996_T-44683_20220916_ICC_IF_22102723_681.webp "RGS4 antibody [HL1522] detects RGS4 protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Unboiled wild-type (WT) and RGS4 knockout (KO) SH-SY5Y cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636996/GTX636996_44732_20230707_WB_KO_watermark_23070301_589.webp "Unboiled wild-type (WT) and RGS4 knockout (KO) SH-SY5Y cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![RGS4 antibody [HL1522] detects RGS4 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636996/GTX636996_44732_20230728_IHC-P_multiple_M_23080901_489.webp "RGS4 antibody [HL1522] detects RGS4 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![RGS4 antibody [HL1522] detects RGS4 protein at cell membrane and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636996/GTX636996_44732_20230728_IHC-P_R_23080901_517.webp "RGS4 antibody [HL1522] detects RGS4 protein at cell membrane and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat intestine. RGS4 stained by RGS4 antibody [HL1522] (GTX636996) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636996/GTX636996_44732_20230915_WB_TPM_watermark_23091901_249.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 antibody [HL1522] (GTX636996) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
RGS4 antibody [HL1522]
GTX636996
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRGS4
Overview
- SupplierGeneTex
- Product NameRGS4 antibody [HL1522]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1522
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5999
- Target nameRGS4
- Target descriptionregulator of G protein signaling 4
- Target synonymsRGP4, SCZD9, regulator of G-protein signaling 4, schizophrenia disorder 9
- HostRabbit
- IsotypeIgG
- Protein IDP49798
- Protein NameRegulator of G-protein signaling 4
- Scientific DescriptionRegulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat Rgs4. This protein negatively regulate signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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