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Rgs6 Polyclonal Antibody

CAC11317
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityRat
TargetRGS6
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Overview

  • Supplier
    Biomatik
  • Product Name
    Rgs6 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9628
  • Target name
    RGS6
  • Target description
    regulator of G protein signaling 6
  • Target synonyms
    GAP, HA117, S914, regulator of G-protein signaling 6, regulator of G-protein signalling 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49758
  • Protein Name
    Regulator of G-protein signaling 6
  • Scientific Description
    The Rgs6 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity
    Rat
  • Reactivity Supplier
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Rat heart tissue All lanes: RGS6 antibody at 8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 57, 54, 50, 51, 53, 52, 41, 56 kDa Observed band size: 55 kDa
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Figure 1. Western blot analysis of RGS6 using anti-RGS6 antibody (A05753-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T47D whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS6 antigen affinity purified polyclonal antibody (Catalog # A05753-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGS6 at approximately 54KD. The expected band size for RGS6 is at 54KD.
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