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RGS7 Antibody

CSB-PA040213
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRGS7
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Overview

  • Supplier
    Cusabio
  • Product Name
    RGS7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6000
  • Target name
    RGS7
  • Target description
    regulator of G protein signaling 7
  • Target synonyms
    regulator of G-protein signaling 7, regulator of G-protein signaling RGS7, regulator of G-protein signalling 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49802
  • Protein Name
    Regulator of G-protein signaling 7
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RGS7 using anti-RGS7 antibody (A04305-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human SK-N-SH whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS7 antigen affinity purified polyclonal antibody (Catalog # A04305-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RGS7 at approximately 65 kDa(h) and 58 kDa(mr). The expected band size for RGS7 is at 58 kDa(h), 55 kDa(m) and 56 kDa(r).
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