![Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Rheb stained by Rheb antibody [GT39810] (GTX634335) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Rheb stained by Rheb antibody [GT39810] (GTX634335) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX634335/GTX634335_42947_20180622_IHC-P_R_w_23061202_927.webp)
Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Rheb stained by Rheb antibody [GT39810] (GTX634335) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Rheb antibody [GT39810]
GTX634335
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRHEB
Overview
- SupplierGeneTex
- Product NameRheb antibody [GT39810]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT39810
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6009
- Target nameRHEB
- Target descriptionRas homolog, mTORC1 binding
- Target synonymsRHEB2, GTP-binding protein Rheb, Ras homolog enriched in brain 2
- HostMouse
- IsotypeIgG2b
- Protein IDQ15382
- Protein NameGTP-binding protein Rheb
- Scientific DescriptionThis gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Rheb antibody [GT39810] (GTX634335) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Rheb antibody [GT39810] (GTX634335) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX634335/GTX634335_42947_20170825_WB_w_23061202_843.webp)
![Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rheb stained by Rheb antibody [GT39810] (GTX634335) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar= 10μm. Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rheb stained by Rheb antibody [GT39810] (GTX634335) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX634335/GTX634335_42947_20180718_ICC_IF_w_23061202_822.webp)
![Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Rheb stained by Rheb antibody [GT39810] (GTX634335) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min Rheb antibody [GT39810] detects Rheb protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Rheb stained by Rheb antibody [GT39810] (GTX634335) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX634335/GTX634335_42947_20180622_IHC-P_M_w_23061202_558.webp)





