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ProductsBack/RHNO1 / C12orf32 Antibody (clone OTI2C12)

RHNO1 / C12orf32 Antibody (clone OTI2C12)

LS-C795913
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetRHNO1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RHNO1 / C12orf32 Antibody (clone OTI2C12)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2C12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID83695
  • Target name
    RHNO1
  • Target description
    RAD9-HUS1-RAD1 interacting nuclear orphan 1
  • Target synonyms
    C12orf32, HKMT1188, RHINO, RAD9, HUS1, RAD1-interacting nuclear orphan protein 1, Rad9, Rad1, Hus1 interacting nuclear orphan, rAD9, RAD1, HUS1-interacting nuclear orphan protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RHNO1 using anti-RHNO1 antibody (A13755). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHNO1 antigen affinity purified polyclonal antibody (Catalog # A13755) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHNO1 at approximately 40 kDa. The expected band size for RHNO1 is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-RHNO1 Antibody Picoband(r)A13755-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot
ReactivityHuman
TargetRHNO1
  • SizePrice