![FACS analysis of HeLa cells using GTX60618 Rho GDI alpha antibody [2G3]. Green : Rho GDI alpha Red : negative control FACS analysis of HeLa cells using GTX60618 Rho GDI alpha antibody [2G3]. Green : Rho GDI alpha Red : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60618/GTX60618_20170912_FACS_w_23061123_775.webp)
FACS analysis of HeLa cells using GTX60618 Rho GDI alpha antibody [2G3]. Green : Rho GDI alpha Red : negative control
Rho GDI alpha antibody [2G3]
GTX60618
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetARHGDIA
Overview
- SupplierGeneTex
- Product NameRho GDI alpha antibody [2G3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2G3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID396
- Target nameARHGDIA
- Target descriptionRho GDP dissociation inhibitor alpha
- Target synonymsGDIA1, HEL-S-47e, NPHS8, RHOGDI, RHOGDI-1, rho GDP-dissociation inhibitor 1, GDP-dissociation inhibitor, aplysia RAS-related 1, Rho GDP dissociation inhibitor (GDI) alpha, epididymis secretory sperm binding protein Li 47e
- HostMouse
- IsotypeIgG1
- Protein IDP52565
- Protein NameRho GDP-dissociation inhibitor 1
- Scientific DescriptionThis gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
- ReactivityHuman, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![ELISA analysis of antigen using GTX60618 Rho GDI alpha antibody [2G3].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng ELISA analysis of antigen using GTX60618 Rho GDI alpha antibody [2G3].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60618/GTX60618_20170912_ELISA_w_23061123_346.webp)
![WB analysis of Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate using GTX60618 Rho GDI alpha antibody [2G3]. WB analysis of Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate using GTX60618 Rho GDI alpha antibody [2G3].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60618/GTX60618_20170912_WB_w_23061123_209.webp)
![ICC/IF analysis of HepG2 cells using GTX60618 Rho GDI alpha antibody [2G3]. Green : Rho GDI alpha Blue: DRAQ5 fluorescent DNA dye ICC/IF analysis of HepG2 cells using GTX60618 Rho GDI alpha antibody [2G3]. Green : Rho GDI alpha Blue: DRAQ5 fluorescent DNA dye](https://www.genetex.com/upload/website/prouct_img/normal/GTX60618/GTX60618_20170912_ICCIF_w_23061123_222.webp)

![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Rho GDI alpha antibody [N1C3] (GTX101428) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101428/GTX101428_39721_20210521_WB_w_23060100_244.webp)
![WB analysis of wild type and Rho GDI alpha knock out 293T cell lysate(10μg per lane) using GTX84859 Rho GDI alpha antibody [1F2]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84859/GTX84859_4673_20181228_WB_w_23061420_107.webp)
![IHC-P analysis of kidney tissue using GTX84860 Rho GDI alpha antibody [1A7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX84860/GTX84860_3180_IHC-P_w_23061420_805.webp)

