Rhodamine-12-dCTP

Biorbyt

Rhodamine-12-dCTP

16

Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: lambdaexc 505 nm, lambdaem 530 nm, epsilon 85.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

1.0-1.1 mM

Liquid

Rhodamine-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Rhodamine-12-dCTP/dCTP ratio for Nick Trans-lation: 30-50% Rhodamine-12-dCTP/70-50% dCTP.

-20°C

12352200