Rhodamine-12-dUTP, Rhodamine-X-(5-aminoallyl)-dUTP, Rho-green-X-dUTP, RhG-dUTP, R110-dUTP

Jena Bioscience

Rhodamine-12-dUTP, Rhodamine-X-(5-aminoallyl)-dUTP, Rho-green-X-dUTP, RhG-dUTP, R110-dUTP

5

Research Use Only

Rhodamine-12-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Rhodamine-12-dUTP/dTTP ratio for Nick Translation: 30-50% Rhodamine-12-dUTP/ 70-50% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

-20°C

41116133