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RHOF Polyclonal Antibody

CAC14412
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetRHOF
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Overview

  • Supplier
    Biomatik
  • Product Name
    RHOF Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54509
  • Target name
    RHOF
  • Target description
    ras homolog family member F, filopodia associated
  • Target synonyms
    ARHF, RIF, rho-related GTP-binding protein RhoF, ras homolog family member F (in filopodia), ras homolog gene family, member F (in filopodia), rho family GTPase Rif, rho in filopodia
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HBH0
  • Protein Name
    Rho-related GTP-binding protein RhoF
  • Scientific Description
    The RHOF Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RHOF using anti-RHOF antibody (A08913-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHOF antigen affinity purified polyclonal antibody (Catalog # A08913-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RHOF at approximately 23KD. The expected band size for RHOF is at 23KD.
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