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WB analysis of human fetal spleen tissue using GTX47387 Ribonuclease A antibody at 0.2-1microg/ml.
WB analysis of human fetal spleen tissue using GTX47387 Ribonuclease A antibody at 0.2-1microg/ml.
WB analysis of human fetal spleen tissue using GTX47387 Ribonuclease A antibody at 0.2-1microg/ml.

Ribonuclease A antibody, N-term

GTX47387
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetRNASE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Ribonuclease A antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6035
  • Target name
    RNASE1
  • Target description
    ribonuclease A family member 1, pancreatic
  • Target synonyms
    HP-RNase; RAC1; RIB1; RIB-1; ribonuclease 1; ribonuclease A C1; ribonuclease pancreatic; ribonuclease, RNase A family, 1 (pancreatic); RNase 1; RNase A; RNase upI-1; RNS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07998
  • Protein Name
    Ribonuclease pancreatic
  • Scientific Description
    This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2,3-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNASE1 using anti-RNASE1 antibody (A03553-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASE1 antigen affinity purified polyclonal antibody (Catalog # A03553-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASE1 at approximately 18 kDa. The expected band size for RNASE1 is at 18 kDa.
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