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ProductsBack/Ribosomal Protein S5 (A-8) FITC

Ribosomal Protein S5 (A-8) FITC

SC-390935FITC
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRPS5
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Ribosomal Protein S5 (A-8) FITC
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    FITC
  • Gene ID6193
  • Target name
    RPS5
  • Target description
    ribosomal protein S5
  • Target synonyms
    S5, uS7, small ribosomal subunit protein uS7, 40S ribosomal protein S5
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPS5 using anti-RPS5 antibody (A04460-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates, Lane 7: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS5 antigen affinity purified polyclonal antibody (Catalog # A04460-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS5 at approximately 23 kDa. The expected band size for RPS5 is at 23 kDa.
Product group Antibodies
Boster Bio
Anti-RPS5 Antibody Picoband(r)A04460-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRPS5
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Product group Antibodies
RayBiotech
Anti-RPS5 Antibody144-06975
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetRPS5
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Product group Antibodies
Biomatik
RPS5 Polyclonal AntibodyCAC14830
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
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Hela cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RNA polymerase 2 CTD repeat YSPTSPS (Ser2) Polyclonal Antibody(bs-6581)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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RNA polymerase II CTD repeat YSPTSPS (phospho S2) Polyclonal AntibodyBS-6581R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Human, Mouse, Rat
TargetRPS5
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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: RPS5 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
Product group Antibodies
Cusabio
RPS5 AntibodyCSB-PA00729A0RB
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TargetRPS5
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RPS5 AntibodyCAB6975
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TargetRPS5
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Immunohistochemical staining of human cerebral cortex, fallopian tube, pancreas and testis using Anti-RPS5 antibody HPA055878 (A) shows similar protein distribution across tissues to independent antibody HPA061979 (B).
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WB analysis of various sample lysates using GTX32851 RPS5 antibody. Dilution : 1:1000 Loading : 25microg per lane
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GeneTex
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TargetRPS5
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Santa Cruz Biotechnology
Ribosomal Protein S5 (A-8): m-IgG Fc BP-HRP BundleSC-526207
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TargetRPS5
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