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ProductsBack/RICK / RIP2 Antibody

RICK / RIP2 Antibody

LS-C748434
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetRIPK2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RICK / RIP2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 45kDa/61kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 45kDa/61kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8767
  • Target name
    RIPK2
  • Target description
    receptor interacting serine/threonine kinase 2
  • Target synonyms
    CARD3, CARDIAK, CCK, GIG30, RICK, RIP2, receptor-interacting serine/threonine-protein kinase 2, CARD-carrying kinase, CARD-containing IL-1 beta ICE-kinase, CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase, RIP-2, growth-inhibiting gene 30, receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase, receptor-interacting protein 2, tyrosine-protein kinase RIPK2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RIP2/RIPK2 using anti-RIP2/RIPK2 antibody (A00818-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP2/RIPK2 antigen affinity purified polyclonal antibody (Catalog # A00818-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP2/RIPK2 at approximately 61 kDa. The expected band size for RIP2/RIPK2 is at 61 kDa.
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