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Western Blot analysis of COS7 cells using FAM80B Polyclonal Antibody
Western Blot analysis of COS7 cells using FAM80B Polyclonal Antibody
Western Blot analysis of COS7 cells using FAM80B Polyclonal Antibody

RIMKLB Antibody

CSB-PA002478
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetRIMKLB
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Overview

  • Supplier
    Cusabio
  • Product Name
    RIMKLB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57494
  • Target name
    RIMKLB
  • Target description
    ribosomal modification protein rimK like family member B
  • Target synonyms
    FAM80B, NAAGS, NAAGS-I, beta-citrylglutamate synthase B, N-acetyl-aspartyl-glutamate synthetase B, N-acetyl-aspartylglutamate synthetase B, N-acetylaspartyl-glutamate synthetase B, NAAG synthetase B, beta-citryl-glutamate synthase B, family with sequence similarity 80, member B, ribosomal protein S6 modification-like protein B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULI2
  • Protein Name
    Beta-citrylglutamate synthase B
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RIMKLB using anti-RIMKLB antibody (A14138-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIMKLB antigen affinity purified polyclonal antibody (Catalog # A14138-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIMKLB at approximately 48 kDa. The expected band size for RIMKLB is at 42 kDa.
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WB analysis of HepG2 and COS cell lysates using GTX87679 RIMKB antibody. The lane on the right is blocked with the synthesized peptide.
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