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WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RIOK1 (Lane 2) using GTX17382 RIOK1 antibody.
WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RIOK1 (Lane 2) using GTX17382 RIOK1 antibody.
WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RIOK1 (Lane 2) using GTX17382 RIOK1 antibody.

RIOK1 antibody

GTX17382
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetRIOK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RIOK1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83732
  • Target name
    RIOK1
  • Target description
    RIO kinase 1
  • Target synonyms
    AD034; bA288G3.1; RIO1; RRP10; serine/threonine-protein kinase RIO1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BRS2
  • Protein Name
    Serine/threonine-protein kinase RIO1
  • Scientific Description
    The protein encoded by this gene competes with pICln for inclusion in the protein arginine methyltransferase 5 complex. This complex targets substrates for dimethylation. The encoded protein is essential for the last steps in the maturation of 40S subunits. [provided by RefSeq, Jan 2017]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RIOK1/RIO1 using anti-RIOK1/RIO1 antibody (A10785-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat testicular tissue lysates, Lane 6: mouse testicular tissue lysates, Lane 7: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIOK1/RIO1 antigen affinity purified polyclonal antibody (Catalog # A10785-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIOK1/RIO1 at approximately 66KD. The expected band size for RIOK1/RIO1 is at 66KD.
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