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ProductsBack/RIOK3 Antibody (aa434-483, HRP)

RIOK3 Antibody (aa434-483, HRP)

LS-C443289
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Equine, Guinea Pig, Hamster, Human, Monkey, Mouse, Primate, Rabbit, Rat, Zebra Fish
TargetRIOK3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RIOK3 Antibody (aa434-483, HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.65 mg/ml
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID8780
  • Target name
    RIOK3
  • Target description
    RIO kinase 3
  • Target synonyms
    SUDD, serine/threonine-protein kinase RIO3, homolog of the Aspergillus nidulans sudD gene product, sudD (suppressor of bimD6, Aspergillus nidulans) homolog, sudD homolog, sudD suppressor of Aspergillus nidulans bimD6 homolog, sudD suppressor of bimD6 homolog, testicular secretory protein Li 47
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Equine, Guinea Pig, Hamster, Human, Monkey, Mouse, Primate, Rabbit, Rat, Zebra Fish
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RIOK3 using anti-RIOK3 antibody (A09262-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIOK3 antigen affinity purified polyclonal antibody (Catalog # A09262-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIOK3 at approximately 65 kDa. The expected band size for RIOK3 is at 59 kDa.
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