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IHC-P analysis of rat kidney tissue using GTX12076 RKIP antibody.
IHC-P analysis of rat kidney tissue using GTX12076 RKIP antibody.
IHC-P analysis of rat kidney tissue using GTX12076 RKIP antibody.

RKIP antibody

GTX12076
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetPebp1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RKIP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. IHC-P: 0.5-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID23980
  • Target name
    Pebp1
  • Target description
    phosphatidylethanolamine binding protein 1
  • Target synonyms
    HCNP, Pbp, Pbp1, Pbpr, Rkip, phosphatidylethanolamine-binding protein 1, HCNPpp, PEBP-1, Raf-1 inhibitor protein, Raf-1 kinase inhibitor protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP70296
  • Protein Name
    Phosphatidylethanolamine-binding protein 1
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Deng Q, Wang Z, Du Y, et al. Transcriptional regulation of PEBP1 expression by androgen receptor in mouse testes. Syst Biol Reprod Med. 2022,68(1):70-79. doi: 10.1080/19396368.2021.2004471
    Read this paper

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Figure 1. Western blot analysis of PEBP1 using anti-PEBP1 antibody (PA1713). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse brian tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEBP1 antigen affinity purified polyclonal antibody (Catalog # PA1713) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEBP1 at approximately 21 kDa. The expected band size for PEBP1 is at 21 kDa.
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