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ProductsBack/RLN1 / Relaxin Antibody (aa25-181)

RLN1 / Relaxin Antibody (aa25-181)

LS-C296209
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRLN1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RLN1 / Relaxin Antibody (aa25-181)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA 0.05-2ug/ml; ICC 5-20ug/ml; WB 0.5-2ug/ml;
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:100 - 1:400) ELISA 0.05-2ug/ml; ICC 5-20ug/ml; WB 0.5-2ug/ml;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.64 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6013
  • Target name
    RLN1
  • Target description
    relaxin 1
  • Target synonyms
    H1, H1RLX, RLXH1, bA12D24.3.1, bA12D24.3.2, prorelaxin H1, preprorelaxin H1
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Relaxin 1 using anti-Relaxin 1 antibody (A08367-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat PC-12 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Relaxin 1 antigen affinity purified polyclonal antibody (Catalog # A08367-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Relaxin 1 at approximately 21KD. The expected band size for Relaxin 1 is at 21KD.
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