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WB analysis of Jurkat (A), Raw264.7 (B), PC12 (C) whole cell lysates using GTX55904 RMP antibody.
WB analysis of Jurkat (A), Raw264.7 (B), PC12 (C) whole cell lysates using GTX55904 RMP antibody.
WB analysis of Jurkat (A), Raw264.7 (B), PC12 (C) whole cell lysates using GTX55904 RMP antibody.

RMP antibody

GTX55904
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetURI1
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Overview

  • Supplier
    GeneTex
  • Product Name
    RMP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8725
  • Target name
    URI1
  • Target description
    URI1 prefoldin like chaperone
  • Target synonyms
    C19orf2; NNX3; PPP1R19; protein phosphatase 1, regulatory subunit 19; RMP; RNA polymerase II subunit 5-mediating protein; RPB5-mediating protein; unconventional prefoldin RPB5 interactor 1; URI
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94763
  • Protein Name
    Unconventional prefoldin RPB5 interactor 1
  • Scientific Description
    This gene encodes member of the prefoldin family of molecular chaperones. The encoded protein functions as a scaffolding protein and plays roles in ubiquitination and transcription, in part though interactions with the RNA polymerase II subunit RPB5. This gene may play a role in multiple malignancies including ovarian cancer and hepatocellular carcinoma. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 22. [provided by RefSeq, Nov 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of C19orf2/URI1 using anti-C19orf2/URI1 antibody (A05245-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human HEL1 whole cell lysates, Lane 5: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C19orf2/URI1 antigen affinity purified polyclonal antibody (Catalog # A05245-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C19orf2/URI1 at approximately 80 kDa. The expected band size for C19orf2/URI1 is at 60 kDa.
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