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ProductsBack/RNase HII-A (G-10) Alexa Fluor(r) 488

RNase HII-A (G-10) Alexa Fluor(r) 488

SC-515475AF488
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetRNASEH2A
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    RNase HII-A (G-10) Alexa Fluor(r) 488
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 488
  • Gene ID10535
  • Target name
    RNASEH2A
  • Target description
    ribonuclease H2 subunit A
  • Target synonyms
    AGS4, JUNB, RNASEHI, RNHIA, RNHL, ribonuclease H2 subunit A, RNase H(35), RNase H2 subunit A, RNase HI large subunit, aicardi-Goutieres syndrome 4 protein, ribonuclease H2, large subunit, ribonuclease HI large subunit, ribonuclease HI subunit A
  • Host
    Mouse
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNASEH2A using anti-RNASEH2A antibody (A07386-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASEH2A antigen affinity purified polyclonal antibody (Catalog # A07386-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASEH2A at approximately 33 kDa. The expected band size for RNASEH2A is at 33 kDa.
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