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Immunohistochemistry of paraffin-embedded Human lung cancer using RNASE6 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using RNASE6 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human lung cancer using RNASE6 Polyclonal Antibody at dilution of 1:100 (40x lens).

RNASE6 Polyclonal Antibody

E-AB-65261
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RNASE6 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a member of the ribonuclease A superfamily and functions in the urinary tract. The protein has broad-spectrum antimicrobial activity against pathogenic bacteria.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNASE6 using anti-RNASE6 antibody (A14127). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASE6 antigen affinity purified polyclonal antibody (Catalog # A14127) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASE6 at approximately 17 kDa. The expected band size for RNASE6 is at 17 kDa.
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