Bio-Connect
Immunofluorescence Microscopy of Goat anti-RING1B antibody (GTX48486). Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25oC. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25oC. Secondary antibody: Alexa FluorR488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.
Immunofluorescence Microscopy of Goat anti-RING1B antibody (GTX48486). Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25oC. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25oC. Secondary antibody: Alexa FluorR488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.
Immunofluorescence Microscopy of Goat anti-RING1B antibody (GTX48486). Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25oC. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25oC. Secondary antibody: Alexa FluorR488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.

RNF2 antibody

GTX48486
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, Other Application
Product group Antibodies
TargetRNF2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    RNF2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:300. ELISA: 1:5000-1:25000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6045
  • Target name
    RNF2
  • Target description
    ring finger protein 2
  • Target synonyms
    BAP-1, BAP1, DING, HIPI3, LUSYAM, RING1B, RING2, E3 ubiquitin-protein ligase RING2, HIP2-interacting protein 3, RING finger protein 1B, RING finger protein BAP-1, RING-type E3 ubiquitin transferase RING2, huntingtin-interacting protein 2-interacting protein 3, protein DinG
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ99496
  • Protein Name
    E3 ubiquitin-protein ligase RING2
  • Scientific Description
    RING1B (also known as BAP1, DING, Polycomb-M33 interacting protein Ring1B, Ring finger protein 1b, Ring finger protein 2 and RNF2) is one of the PcG proteins. The polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme that possesses ubiquitin ligase activity.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203