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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA736198LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA736198LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA736198LA01HU at dilution of 1:100

RNF20 Antibody

CSB-PA736198LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetRNF20
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Overview

  • Supplier
    Cusabio
  • Product Name
    RNF20 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56254
  • Target name
    RNF20
  • Target description
    ring finger protein 20
  • Target synonyms
    BRE1; BRE1 E3 ubiquitin ligase homolog; BRE1A; E3 ubiquitin-protein ligase BRE1A; hBRE1; homolog of S. cerevisiae BRE1; RING-type E3 ubiquitin transferase BRE1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VTR2
  • Protein Name
    E3 ubiquitin-protein ligase BRE1A
  • Scientific Description
    Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of Lys-120 of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 Lys-4 and Lys-79 methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RNF20 using anti-RNF20 antibody (A03457-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF20 antigen affinity purified polyclonal antibody (Catalog # A03457-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF20 at approximately 114 kDa. The expected band size for RNF20 is at 114 kDa.
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