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RNH1 antibody

10R-1151
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    RNH1 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNH1 using anti-RNH1 antibody (PB9885). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SK-OV-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: human SW620 whole cell lysates, Lane 8: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNH1 antigen affinity purified polyclonal antibody (Catalog # PB9885) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNH1 at approximately 50KD. The expected band size for RNH1 is at 50KD.
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The extracts of mouse liver (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RNH1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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