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RNMT Antibody

CSB-PA019902GA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRNMT
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Overview

  • Supplier
    Cusabio
  • Product Name
    RNMT Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8731
  • Target name
    RNMT
  • Target description
    RNA guanine-7 methyltransferase
  • Target synonyms
    CMT1, CMT1c, MET, Met, N7-MTase, RG7MT1, cm1p, hCMT1, hMet, mRNA cap guanine-N(7) methyltransferase, RNA (guanine-7-) methyltransferase, hcm1p, mRNA (guanine-7-)methyltransferase, mRNA (guanine-N(7)-)-methyltransferase, mRNA cap guanine-N7 methyltransferase, mRNA cap methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43148
  • Protein Name
    mRNA cap guanine-N7 methyltransferase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RNMT using anti-RNMT antibody (A07752-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNMT antigen affinity purified polyclonal antibody (Catalog # A07752-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNMT at approximately 56 kDa. The expected band size for RNMT is at 55 kDa.
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