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ProductsBack/RNPEP Antibody (clone OTI1B1, Carrier-free)

RNPEP Antibody (clone OTI1B1, Carrier-free)

LS-C797549
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetRNPEP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RNPEP Antibody (clone OTI1B1, Carrier-free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:150), WB (1:500) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1B1
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6051
  • Target name
    RNPEP
  • Target description
    arginyl aminopeptidase
  • Target synonyms
    AP-B, APB, aminopeptidase B, arginine aminopeptidase
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AP-B/RNPEP using anti-AP-B/RNPEP antibody (A10683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: mouse small intestine tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP-B/RNPEP antigen affinity purified polyclonal antibody (Catalog # A10683-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP-B/RNPEP at approximately 73 kDa. The expected band size for AP-B/RNPEP is at 73 kDa.
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