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WB analysis of U-87MG cell lysate using GTX33470 ROR2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of U-87MG cell lysate using GTX33470 ROR2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of U-87MG cell lysate using GTX33470 ROR2 antibody. Dilution : 1:1000 Loading : 25microg per lane

ROR2 antibody

GTX33470
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetROR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    ROR2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4920
  • Target name
    ROR2
  • Target description
    receptor tyrosine kinase like orphan receptor 2
  • Target synonyms
    BDB; BDB1; neurotrophic tyrosine kinase receptor-related 2; NTRKR2; tyrosine-protein kinase transmembrane receptor ROR2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01974
  • Protein Name
    Tyrosine-protein kinase transmembrane receptor ROR2
  • Scientific Description
    The protein encoded by this gene is a receptor protein tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. The protein may be involved in the early formation of the chondrocytes and may be required for cartilage and growth plate development. Mutations in this gene can cause brachydactyly type B, a skeletal disorder characterized by hypoplasia/aplasia of distal phalanges and nails. In addition, mutations in this gene can cause the autosomal recessive form of Robinow syndrome, which is characterized by skeletal dysplasia with generalized limb bone shortening, segmental defects of the spine, brachydactyly, and a dysmorphic facial appearance. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ROR2 using anti-ROR2 antibody (A01840-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat small intestine tissue lysates, Lane 6: rat ovary tissue lysates, Lane 8: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROR2 antigen affinity purified polyclonal antibody (Catalog # A01840-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROR2 at approximately 105 kDa. The expected band size for ROR2 is at 105 kDa.
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