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ProductsBack/RPA 70 kDa subunit (B-6): m-IgGkappa BP-HRP Bundle

RPA 70 kDa subunit (B-6): m-IgGkappa BP-HRP Bundle

SC-520788
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRPA1
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    RPA 70 kDa subunit (B-6): m-IgGkappa BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6117
  • Target name
    RPA1
  • Target description
    replication protein A1
  • Target synonyms
    HSSB, MST075, PFBMFT6, REPA1, RF-A, RP-A, RPA70, replication protein A 70 kDa DNA-binding subunit, MSTP075, RF-A protein 1, RP-A p70, replication factor A protein 1, replication protein A1, 70kDa, single-stranded DNA-binding protein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPA70 using anti-RPA70 antibody (PB9886). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA70 antigen affinity purified polyclonal antibody (Catalog # PB9886) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPA70 at approximately 70 kDa. The expected band size for RPA70 is at 68 kDa.
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