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The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA092850(RPAP3 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA092850(RPAP3 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA092850(RPAP3 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)

RPAP3 Antibody

CSB-PA092850
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRPAP3
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPAP3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79657
  • Target name
    RPAP3
  • Target description
    RNA polymerase II associated protein 3
  • Target synonyms
    hSpagh; RNA polymerase II-associated protein 3; Tah1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H6T3
  • Protein Name
    RNA polymerase II-associated protein 3
  • Scientific Description
    This gene encodes an RNA polymerase II-associated protein. The encoded protein may function in transcriptional regulation and may also regulate apoptosis. Alternatively spliced transcript variants have been described. Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Immunohistochemical staining of human cerebral cortex, colon, fallopian tube and testis using Anti-RPAP3 antibody HPA038311 (A) shows similar protein distribution across tissues to independent antibody HPA038312 (B).
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Figure 1. Western blot analysis of RPAP3 using anti-RPAP3 antibody (A03391-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPAP3 antigen affinity purified polyclonal antibody (Catalog # A03391-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPAP3 at approximately 76 kDa. The expected band size for RPAP3 is at 76 kDa.
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