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ProductsBack/RPB8 antibody [N1C3]

RPB8 antibody [N1C3]

GTX114917
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetPOLR2H
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Overview

  • Supplier
    GeneTex
  • Product Name
    RPB8 antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.48 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5437
  • Target name
    POLR2H
  • Target description
    RNA polymerase II, I and III subunit H
  • Target synonyms
    DNA-directed RNA polymerase II subunit H; DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; DNA-directed RNA polymerases I, II, and III subunit RPABC3; polymerase (RNA) II (DNA directed) polypeptide H; polymerase (RNA) II subunit H; RNA polymerase II subunit H; RPABC3; RPB17; RPB8; RPB8 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52434
  • Protein Name
    DNA-directed RNA polymerases I, II, and III subunit RPABC3
  • Scientific Description
    This gene encodes one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of POLR2H using anti-POLR2H antibody (A11270-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2H antigen affinity purified polyclonal antibody (Catalog # A11270-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLR2H at approximately 17 kDa. The expected band size for POLR2H is at 17 kDa.
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