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RPE Antibody

LS-C749760
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRPE
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RPE Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 17kDa/19kDa/21kDa/23kDa/24kDa, while the observed MW by Western blot was 25kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 17kDa/19kDa/21kDa/23kDa/24kDa, while the observed MW by Western blot was 25kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6120
  • Target name
    RPE
  • Target description
    ribulose-5-phosphate-3-epimerase
  • Target synonyms
    ribulose-phosphate 3-epimerase; RPE2-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPE using anti-RPE antibody (A05272-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPE antigen affinity purified polyclonal antibody (Catalog # A05272-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPE at approximately 30 kDa. The expected band size for RPE is at 25 kDa.
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