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Western Blot analysis of customers (cat sample) using QM Polyclonal Antibody.
Western Blot analysis of customers (cat sample) using QM Polyclonal Antibody.
Western Blot analysis of customers (cat sample) using QM Polyclonal Antibody.

RPL10 Antibody

CSB-PA040237
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRPL10
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPL10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6134
  • Target name
    RPL10
  • Target description
    ribosomal protein L10
  • Target synonyms
    AUTSX5, DXS648, DXS648E, L10, MRXS35, NOV, QM, uL16, large ribosomal subunit protein uL16, 60S ribosomal protein L10, Wilms tumor-related protein, laminin receptor homolog, tumor suppressor QM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27635
  • Protein Name
    60S ribosomal protein L10
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPL10 using anti-RPL10 antibody (A04190-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL10 antigen affinity purified polyclonal antibody (Catalog # A04190-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL10 at approximately 23 kDa. The expected band size for RPL10 is at 23 kDa.
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