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Western Blot analysis of COS7 cells using Ribosomal Protein L17 Polyclonal Antibody
Western Blot analysis of COS7 cells using Ribosomal Protein L17 Polyclonal Antibody
Western Blot analysis of COS7 cells using Ribosomal Protein L17 Polyclonal Antibody

RPL17 Antibody

CSB-PA003980
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetRPL17
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPL17 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6139
  • Target name
    RPL17
  • Target description
    ribosomal protein L17
  • Target synonyms
    60S ribosomal protein L17; 60S ribosomal protein L23; gene encoding putative NFkB activating protein; L17; large ribosomal subunit protein uL22; PD-1; RPL23
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18621
  • Protein Name
    60S ribosomal protein L17
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPL17 using anti-RPL17 antibody (A06980-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL17 antigen affinity purified polyclonal antibody (Catalog # A06980-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL17 at approximately 21 kDa. The expected band size for RPL17 is at 21 kDa.
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