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Western Blot analysis of COLO205 cells using Ribosomal Protein L18 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Ribosomal Protein L18 Polyclonal Antibody
Western Blot analysis of COLO205 cells using Ribosomal Protein L18 Polyclonal Antibody

RPL18 Antibody

CSB-PA003981
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRPL18
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPL18 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6141
  • Target name
    RPL18
  • Target description
    ribosomal protein L18
  • Target synonyms
    60S ribosomal protein L18; DBA18; L18; large ribosomal subunit protein eL18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07020
  • Protein Name
    60S ribosomal protein L18
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPL18 using anti-RPL18 antibody (A07057-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL18 antigen affinity purified polyclonal antibody (Catalog # A07057-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL18 at approximately 23 kDa. The expected band size for RPL18 is at 22 kDa.
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