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ProductsBack/RPL23 / Ribosomal Protein L23 Antibody (HRP)

RPL23 / Ribosomal Protein L23 Antibody (HRP)

LS-C766144
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetRPL23
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RPL23 / Ribosomal Protein L23 Antibody (HRP)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:2000 - 1:5000(Enhancedchemiluminescentdetection)WB1:300) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID9349
  • Target name
    RPL23
  • Target description
    ribosomal protein L23
  • Target synonyms
    60S ribosomal protein L17; 60S ribosomal protein L23; L23; large ribosomal subunit protein uL14; ribosomal protein L17; rpL17
  • Host
    Donkey
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPL23 using anti-RPL23 antibody (A06155-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL23 antigen affinity purified polyclonal antibody (Catalog # A06155-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL23 at approximately 17 kDa. The expected band size for RPL23 is at 17 kDa.
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