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RPL5 (C) Antibody, Rabbit Polyclonal

ORB67036
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
TargetRPL5
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Overview

  • Supplier
    Biorbyt
  • Product Name
    RPL5 antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB:1:1000-1:5000 WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6125
  • Target name
    RPL5
  • Target description
    ribosomal protein L5
  • Target synonyms
    60S ribosomal protein L5; L5; large ribosomal subunit protein uL18; MSTP030; PPP1R135; protein phosphatase 1, regulatory subunit 135; uL18
  • Host
    Rabbit
  • Protein IDP46777
  • Protein Name
    60S ribosomal protein L5
  • Scientific Description
    Rabbit polyclonal to RPL5.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large60S subunit. Together these subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. RPL5 is a component of the 60S subunit that belongs to the L18P familyof ribosomal proteins. RPL5 is located in the cytoplasm and binds 5S rRNA to form a stable complexcalled the 5S ribonucleoprotein particle NP), which is necessary for the transport of nonribosomeassociatedcytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. RPL5 interactsspecifically with the beta subunit of casein kinase II. Variable expression of RPL5 in colorectalcancers compared to adjacent normal tissues has been observed, although no correlation betweenthe level of expression and the severity of the disease has been found.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPL5 using anti-RPL5 antibody (A02843-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL5 antigen affinity purified polyclonal antibody (Catalog # A02843-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL5 at approximately 35 kDa. The expected band size for RPL5 is at 35 kDa.
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