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Western Blot Positive WB detected in: RAW264.7 whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate All lanes: RPL7A antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
Western Blot Positive WB detected in: RAW264.7 whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate All lanes: RPL7A antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
Western Blot Positive WB detected in: RAW264.7 whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate All lanes: RPL7A antibody at 5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa

RPL7A Antibody

CSB-PA020303LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRPL7A
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPL7A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6130
  • Target name
    RPL7A
  • Target description
    ribosomal protein L7a
  • Target synonyms
    L7A, SURF3, TRUP, eL8, large ribosomal subunit protein eL8, 60S ribosomal protein L7a, PLA-X polypeptide, surfeit 3, surfeit locus protein 3, thyroid hormone receptor uncoupling protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62424
  • Protein Name
    Large ribosomal subunit protein eL8
  • Scientific Description
    cytoplasm, cytosol, cytosolic large ribosomal subunit, extracellular exosome, focal adhesion, membrane, nucleolus, nucleus, polysomal ribosome, cadherin binding
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPL7A using anti-RPL7A antibody (M08246-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat pancreas tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse pancreas tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL7A antigen affinity purified monoclonal antibody (Catalog # M08246-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL7A at approximately 30 kDa. The expected band size for RPL7A is at 30 kDa.
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