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Western Blot analysis of Hela and A549 cell, Human liver cancer tissue using RPN1 Polyclonal Antibody at dilution of 1:350
Western Blot analysis of Hela and A549 cell, Human liver cancer tissue using RPN1 Polyclonal Antibody at dilution of 1:350
Western Blot analysis of Hela and A549 cell, Human liver cancer tissue using RPN1 Polyclonal Antibody at dilution of 1:350

RPN1 Polyclonal Antibody

E-AB-10882
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    RPN1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
  • UNSPSC
    12352203

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Western blot All lanes: RPN1 antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa
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Figure 1. Western blot analysis of Ribophorin I/RPN1 using anti-Ribophorin I/RPN1 antibody (A05063-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ribophorin I/RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ribophorin I/RPN1 at approximately 68 kDa. The expected band size for Ribophorin I/RPN1 is at 68 kDa.
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