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Anti-RPN1 Antibody

CAB6726
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetRPN1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-RPN1 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID6184
  • Target name
    RPN1
  • Target description
    ribophorin I
  • Target synonyms
    OST1, RBPH1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, RPN-I, dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit, oligosaccharyltransferase 1 homolog, oligosaccharyltransferase complex subunit (non-catalytic), ribophorin-1
  • Host
    Rabbit
  • Protein IDP04843
  • Protein Name
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
  • Scientific Description
    RPN1 Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Ribophorin I/RPN1 using anti-Ribophorin I/RPN1 antibody (A05063-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ribophorin I/RPN1 antigen affinity purified polyclonal antibody (Catalog # A05063-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ribophorin I/RPN1 at approximately 68 kDa. The expected band size for Ribophorin I/RPN1 is at 68 kDa.
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