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ProductsBack/RPS17 / Ribosomal Protein S17 Antibody (N-Terminus)

RPS17 / Ribosomal Protein S17 Antibody (N-Terminus)

LS-C353872
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRPS17
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RPS17 / Ribosomal Protein S17 Antibody (N-Terminus)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6218
  • Target name
    RPS17
  • Target description
    ribosomal protein S17
  • Target synonyms
    DBA4, RPS17L, RPS17L1, RPS17L2, S17, eS17, small ribosomal subunit protein eS17, 40S ribosomal protein S17
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPS17 using anti-RPS17 antibody (A05831-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS17 antigen affinity purified polyclonal antibody (Catalog # A05831-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS17 at approximately 18 kDa. The expected band size for RPS17 is at 16 kDa.
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