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ProductsBack/RPS6KA5 / MSK1 Antibody (phospho-Thr581)

RPS6KA5 / MSK1 Antibody (phospho-Thr581)

LS-C822025
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetRPS6KA5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RPS6KA5 / MSK1 Antibody (phospho-Thr581)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA (1:40000), IF (1:100 - 1:500), WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9252
  • Target name
    RPS6KA5
  • Target description
    ribosomal protein S6 kinase A5
  • Target synonyms
    MSK1, MSPK1, RLPK, ribosomal protein S6 kinase alpha-5, 90 kDa ribosomal protein S6 kinase 5, RSK-like protein kinase, RSKL, S6K-alpha-5, nuclear mitogen- and stress-activated protein kinase 1, ribosomal protein S6 kinase, 90kDa, polypeptide 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MSK1 using anti-MSK1 antibody (PB9268). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSK1 antigen affinity purified polyclonal antibody (Catalog # PB9268) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSK1 at approximately 90 kDa. The expected band size for MSK1 is at 90 kDa.
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