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ProductsBack/phospho-RPS6KB1 (Thr412) Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with RPS6KB1(Thr 412) Polyclonal Antibody (bs-5669R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with RPS6KB1(Thr 412) Polyclonal Antibody (bs-5669R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with RPS6KB1(Thr 412) Polyclonal Antibody (bs-5669R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

phospho-RPS6KB1 (Thr412) Polyclonal Antibody

BS-5669R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetRPS6KB1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    RPS6KB1(Thr 412) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6198
  • Target name
    RPS6KB1
  • Target description
    ribosomal protein S6 kinase B1
  • Target synonyms
    p70 S6KA; p70(S6K)-alpha; p70-alpha; p70-S6K; PS6K; ribosomal protein S6 kinase beta-1; ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kDa, polypeptide 1; S6K; S6K1; S6K-beta-1; serine/threonine kinase 14 alpha; serine/threonine-protein kinase 14A; STK14A
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Switch to low-fat diet improves outcome of acute lymphoblastic leukemia in obese mice. Tucci J et al., 2018, Cancer Metab
    Read more

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Figure 1. Western blot analysis of S6K1/RPS6KB1 using anti-S6K1/RPS6KB1 antibody (A01475-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT4 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S6K1/RPS6KB1 antigen affinity purified polyclonal antibody (Catalog # A01475-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S6K1/RPS6KB1 at approximately 59 kDa. The expected band size for S6K1/RPS6KB1 is at 59 kDa.
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