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Western Blot analysis of 293T cells using p70 S6 kinase alpha Polyclonal Antibody
Western Blot analysis of 293T cells using p70 S6 kinase alpha Polyclonal Antibody
Western Blot analysis of 293T cells using p70 S6 kinase alpha Polyclonal Antibody

RPS6KB1 Antibody

CSB-PA003686
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRPS6KB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RPS6KB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6198
  • Target name
    RPS6KB1
  • Target description
    ribosomal protein S6 kinase B1
  • Target synonyms
    PS6K, S6K, S6K-beta-1, S6K1, STK14A, p70 S6KA, p70(S6K)-alpha, p70-S6K, p70-alpha, ribosomal protein S6 kinase beta-1, ribosomal protein S6 kinase I, ribosomal protein S6 kinase, 70kDa, polypeptide 1, serine/threonine kinase 14 alpha, serine/threonine-protein kinase 14A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23443
  • Protein Name
    Ribosomal protein S6 kinase beta-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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This image was generously provided by Piotr Mamczur at University of Wroclaw as part of the Bioss Discovery Program. Mouse squamous cell carcinoma (KLN-205 cell line) were probed with Rabbit Anti-RPS6KB1 Polyclonal Antibody (bs-1426R) at 1:100 followed by at FITC conjugated secondary antibody and DAPI staining
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Figure 1. Western blot analysis of S6K1/RPS6KB1 using anti-S6K1/RPS6KB1 antibody (A01475-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT4 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S6K1/RPS6KB1 antigen affinity purified polyclonal antibody (Catalog # A01475-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S6K1/RPS6KB1 at approximately 59 kDa. The expected band size for S6K1/RPS6KB1 is at 59 kDa.
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