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RPTOR Antibody

ABX302159
Abbexa
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
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Product group Antibodies
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Overview

  • Supplier
    Abbexa
  • Product Name
    RPTOR Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Raptor/RPTOR using anti-Raptor/RPTOR antibody (A01463-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Mcf-7 whole cell lysates, Lane 4: human Hek293 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Raptor/RPTOR antigen affinity purified polyclonal antibody (Catalog # A01463-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Raptor/RPTOR at approximately 150KD. The expected band size for Raptor/RPTOR is at 150KD.
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Paraformaldehyde-fixed, paraffin embedded mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Raptor Polyclonal Antibody, Unconjugated (bs-3559R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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