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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA083779(RRBP1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA083779(RRBP1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA083779(RRBP1 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)

RRBP1 Antibody

CSB-PA083779
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRRBP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RRBP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6238
  • Target name
    RRBP1
  • Target description
    ribosome binding protein 1
  • Target synonyms
    ES/130, ES130, RRp, hES, p180, ribosome-binding protein 1, 180 kDa ribosome receptor homolog, ES/130-related protein, endoplasmic reticulum ribosome-binding protein 1, ribosomal receptor-binding protein 1, ribosome binding protein 1 homolog 180kDa (dog), ribosome receptor protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P2E9
  • Protein Name
    Ribosome-binding protein 1
  • Scientific Description
    This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of RRBP1 using anti-RRBP1 antibody (A07074-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRBP1 antigen affinity purified polyclonal antibody (Catalog # A07074-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRBP1 at approximately 200KD. The expected band size for RRBP1 is at 152KD.
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