Western blot using GeneTex's affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kDa, indicated by the arrowhead, corresponds to PBK1.
Western blot using GeneTex's affinity purified anti-PBK1 antibody shows detection of over-expressed PBK1 in lysates from HeLa cells transfected with Flag-PBK1. Lanes 1 and 3 contain lysate from Flag-PBK1 transfected HeLa cells. Lanes 2 and 4 contain lysate from cells transfected with vector. Lanes 1 and 2 were blotted with anti-Flag antibody. Lanes 3 and 4 were probed with a 1:500 dilution of anti-PBK1. The band at 75 kDa, indicated by the arrowhead, corresponds to PBK1.
RSL1D1 antibody
GTX48683
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetRSL1D1
Overview
- SupplierGeneTex
- Product NameRSL1D1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ICC/IF: 1:100. IP: 1:100. ELISA: 1:2500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.1 mg/ml
- ConjugateUnconjugated
- Gene ID26156
- Target nameRSL1D1
- Target descriptionribosomal L1 domain containing 1
- Target synonymsCSIG, L12, PBK1, UTP30, ribosomal L1 domain-containing protein 1, CATX-11, cellular senescence-inhibited gene protein
- HostRabbit
- IsotypeIgG
- Scientific DescriptionPBK1 protein (also known as Ribosomal L1 domain-containing protein 1, cellular senescence-inhibited gene protein, and CATX-11) was isolated from highly invasive first trimester trophoblast cells and has been proposed to regulate their naturally occurring invasive behavior (Huch et al., 1998). PBK1 was also found to be over-expressed in non-small-cell lung cancer (NSCLC) cells (Petroziello et al., 2004).A recent study suggests that PBK1 may up-regulate the urokinase-type plasminogen activator (uPA) gene, which plays an important role in cellular matrix degradation and
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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