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Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, HEK293 whole cell lysate All lanes: RTCB antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, HEK293 whole cell lysate All lanes: RTCB antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, HEK293 whole cell lysate All lanes: RTCB antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa

RTCB Antibody

CSB-PA897546LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetRTCB
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Overview

  • Supplier
    Cusabio
  • Product Name
    RTCB Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51493
  • Target name
    RTCB
  • Target description
    RNA 2',3'-cyclic phosphate and 5'-OH ligase
  • Target synonyms
    3'-phosphate/5'-hydroxy nucleic acid ligase; ankyrin repeat domain 54; C22orf28; DJ149A16.6; FAAP; focal adhesion-associated protein; HSPC117; RNA-splicing ligase RtcB homolog; tRNA-splicing ligase RtcB homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y3I0
  • Protein Name
    tRNA-splicing ligase RtcB homolog
  • Scientific Description
    Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3,5-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RTCB using anti-RTCB antibody (A05076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (Catalog # A05076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.
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