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RTL1 Antibody

CSB-PA881930XA01DOA
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityPlant
TargetRTL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    RTL1 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID844404
  • Target name
    RTL1
  • Target description
    RNAse THREE-like protein 1
  • Target synonyms
    AT1G80650, RNAse THREE-like protein 1, T21F11.2, T21F11_2, RNAse THREE-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9M8N2
  • Protein Name
    Ribonuclease 3-like protein 1
  • Reactivity
    Plant
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RTL1 using anti-RTL1 antibody (A11807). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTL1 antigen affinity purified polyclonal antibody (Catalog # A11807) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTL1 at approximately 200 kDa. The expected band size for RTL1 is at 155 kDa.
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